Environmental Toxicology 27(4)
Overview of new articles on POPs in a new issue of the Environmental Toxicology journal.
Benzo(a)pyrene induces p73 mRNA expression and necrosis in human lung adenocarcinoma H1299 cells
pages 202–210
Ying Jiang, Kaimin Rao, Guangtao Yang, Xi Chen, Qian Wang, Ailin Liu, Hongyan Zheng and Jing Yuan
- p53 can mediate DNA damage-induced apoptosis in various cell lines treated with Benzo(a)pyrene (BaP). However, the potential role of p73, one of the p53 family members, in BaP-induced apoptotic cell death remains to be determined. In this study, normal fetal lung fibroblasts (MRC-5) and human lung adenocarcinoma cells (H1299, p53-null) were treated with BaP at concentrations of 8, 16, 32, 64, and 128 μM for 4 and 12 h. The oxidative stress status, extent of DNA damage, expression of p53, p73, mdm2, bcl-2, and bax at the mRNA and protein levels, and the percentages of apoptosis and/or necrosis were assessed.
pages 238–243
Juan Manuel Parra, Sebastián Sánchez-Fortún and Argelia Castaño
- The cytotoxic and genotoxic effects induced by alachlor and dichlorvos, two pesticides broadly used, were studied on RTG-2 fish cell line. As measure of cytotoxicity, neutral red assay was used to determine the cellular viability. Toxicity ranking based on IC50 values found that alachlor was more cytotoxic than dichlorvos.
13.3.2012
